PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||50662||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
- Total vector size (bp) 7500
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Insert Size (bp)450
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer cgggtttattacagggacagcag
- 3′ sequencing primer taccagtcaatctttcacaaattttgt (Common Sequencing Primers)
For more information on OE-PCR please see: http://en.wikipedia.org/wiki/Overlap_extension_polymerase_chain_reaction)
gRNA target sequence GGGGCCACTAGGGACAGGAT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLX-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 50662)
For your References section:Genetic screens in human cells using the CRISPR-Cas9 system. Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. 10.1126/science.1246981 PubMed 24336569
Generated by Addgene from full sequence supplied by depositor.