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Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
Daringer NM, Dudek RM, Schwarz KA, Leonard JN
ACS Synth Biol. 2014 Mar 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58854tTA_BFPConstitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901)
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58855pBI-MCS-EYFPThe tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP
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58856mCherry_LD 0_CS G_tTA-BFPMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
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58868mCherry_LD 0_TEVMESA protease chain with mCherry ectodomain and no linker domain
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58869CD4_LD 0_CS G_tTA-BFPMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
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58870CD4_LD 0_TEVMESA protease chain with CD4 ectodomain and no linker domain
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58871dTomato_LD 0_CS G_tTA-BFPMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
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58872dTomato_LD 0_TEVMESA protease chain with dTomato ectodomain and no linker domain
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58873mCherry_LD 0_CS A_tTA-BFPMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion
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58874mCherry_LD 0_CS M_tTA-BFPMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
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58875dTomato_LD 0_CS M_tTA-BFPMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
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58876FRB_LD 0_CS G_tTA-BFPMESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion
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58877FKBP_LD 0_TEVMESA protease chain with Rapamycin FKBP ectodomain and no linker domain
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58878FKBP_SCF2_LD6_NTEVMESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease
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58879FRB_SCF2_LD6_CTEVMESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease
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