Modular Extracellular Sensor Architecture for Engineering Mammalian Cell-based Devices.
Daringer NM, Dudek RM, Schwarz KA, Leonard JN
ACS Synth Biol. 2014 Mar 11. PubMed Article
Daringer NM, Dudek RM, Schwarz KA, Leonard JN
ACS Synth Biol. 2014 Mar 11. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 58854 | tTA_BFP | Constitutive expression of tTA-EBFP fusion protein (tTA from CL-CTIG, Addgene plasmid 14901) |
| 58855 | pBI-MCS-EYFP | The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP |
| 58856 | mCherry_LD 0_CS G_tTA-BFP | MESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58868 | mCherry_LD 0_TEV | MESA protease chain with mCherry ectodomain and no linker domain |
| 58869 | CD4_LD 0_CS G_tTA-BFP | MESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58870 | CD4_LD 0_TEV | MESA protease chain with CD4 ectodomain and no linker domain |
| 58871 | dTomato_LD 0_CS G_tTA-BFP | MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58872 | dTomato_LD 0_TEV | MESA protease chain with dTomato ectodomain and no linker domain |
| 58873 | mCherry_LD 0_CS A_tTA-BFP | MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion |
| 58874 | mCherry_LD 0_CS M_tTA-BFP | MESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion |
| 58875 | dTomato_LD 0_CS M_tTA-BFP | MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion |
| 58876 | FRB_LD 0_CS G_tTA-BFP | MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion |
| 58877 | FKBP_LD 0_TEV | MESA protease chain with Rapamycin FKBP ectodomain and no linker domain |
| 58878 | FKBP_SCF2_LD6_NTEV | MESA split-TEV protease chain with Rapamycin FKBP ectodomain, 2 aa scaffold, 6 aa linker domain, N-terminal TEV protease |
| 58879 | FRB_SCF2_LD6_CTEV | MESA split-TEV protease chain with Rapamycin FRB ectodomain 2 aa scaffold, 6 aa linker domain, C-terminal TEV protease |