Tumor suppressor miR-375 regulates MYC expression via repression of CIP2A coding sequence through multiple miRNA-mRNA interactions.
Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang LJ, Yang LJ, Chan EK
Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. PubMed Article
Jung HM, Patel RS, Phillips BL, Wang H, Cohen DM, Reinhold WC, Chang LJ, Yang LJ, Chan EK
Mol Biol Cell. 2013 Jun;24(11):1638-48, S1-7. doi: 10.1091/mbc.E12-12-0891. Epub 2013 Apr 3. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 53705 | pMiR-CIP2A(partial) | Luciferase reporter assay for microRNA binding on CIP2A coding sequence |
| 53706 | pMiR-CIP2A-miR-375-mutA | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site |
| 53707 | pMiR-CIP2A-miR-375-mutB | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site |
| 53708 | pMiR-CIP2A-miR-375-mutC | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site |
| 53709 | pMiR-CIP2A-miR-375-mutD | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site |
| 53710 | pMiR-CIP2A-miR-375-mutE | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding site |
| 53711 | pMiR-CIP2A-miR-375-mutA&B | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites |
| 53712 | pMiR-CIP2A-miR-375-mutC&D | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites |
| 53713 | pMiR-CIP2A-stop-mut | Luciferase reporter assay for CIP2A with mutated firefly stop codon |
| 53714 | pMiR-CIP2A-stop-mut-miR-375-mutA-E | Luciferase reporter assay for CIP2A with mutated firefly stop codon that also has five miR-375 binding site mutations |
| 53754 | pMiR-CIP2A-miR-375-mutA-E | Luciferase reporter assay for CIP2A with point mutation on miR-375 binding sites |