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Activation of prokaryotic transcription through arbitrary protein-protein contacts.
Dove SL, Joung JK, Hochschild A
Nature. 1997 Apr 10;386(6625):627-30.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
53730pACλCIPlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.
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53731pBRαPlpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
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53732pBRα-σ70 D581GPlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
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53733pACλCI-β-flap (831-1057)Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
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53734pBRα-β flap (831-1057)Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.
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53735FW102 OL2-62Bacterial-two hybrid reporter strain
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