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Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells.
Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK
Mol Cell. 2014 May 14. pii: S1097-2765(14)00355-4. doi: 10.1016/j.molcel.2014.04.022.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
55195CMVp-dCas9-3xNLS-VP64 (Construct 1)Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.
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55196PGK1p-Csy4-pA (Construct 2)Expresses Csy4 in Mammalian cells for site-specific RNA processing
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55197P1-EYFP-pA (Construct 5)Synthetic promoter responsive to gRNA1
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55198P2-ECFP-pA (Construct 6)Synthetic promoter responsive to gRNA2
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55199CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)CFP reporter along with FF4 miRNA binding sites and 28 nt sequences
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55200CMVp-dsRed2-Triplex-28-gRNA1-28-pAPlasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
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55201CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pAPlasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
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55202CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pAPlasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
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