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Improved vectors and genome-wide libraries for CRISPR screening.
Sanjana NE, Shalem O, Zhang F
Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
52961lentiCRISPR v2Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
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52962lentiCas9-BlastExpresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.
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52963lentiGuide-PuroExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.
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63593lentiCas9n(D10A)-BlastExpresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
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