Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

CRISPR-Cas9-based Photoactivatable Transcription System.
Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
64119NLS-dCas9-trCIB1Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1
Loading...
64124NLSx3-CRY2PHR-p65Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.
Loading...
64125GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.
Loading...
64126pNeurog2-Luciferase reporterPhotoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.
Loading...
64127Tet-inducible Luciferase reporterPhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.
Loading...
64128Tet-inducible mCherry reporterPhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter
Loading...
64129sgRNA1_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.
Loading...
64130sgRNA2_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.
Loading...
64131sgRNA3_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette.
Loading...
64132sgRNA4_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette.
Loading...
64136sgRNA1_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.
Loading...
64137sgRNA2_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.
Loading...
64138sgRNA3_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64139sgRNA4_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64140sgRNA1_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64142sgRNA2_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64151sgRNA3_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64152sgRNA4_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64153sgRNA1_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64154sgRNA2_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64155sgRNA3_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64156sgRNA4_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64157sgRNA1_GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64158sgRNA2_GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64159sgRNA3_GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
Loading...
64160sgRNA1_pNeurog2-Luciferase reporterPhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.
Loading...
64161sgRNA1_Tet-inducible Luciferase reporterPhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.
Loading...

Antibodies from Article