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CRISPR-Cas9-based Photoactivatable Transcription System.
Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
Chem Biol. 2015 Feb 19;22(2):169-74. doi: 10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
64119NLS-dCas9-trCIB1Photoactivatable transcription system. Expression of genomic anchor probe, containing dCas9 and CIB1
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64124NLSx3-CRY2PHR-p65Photoactivatable transcription system. Expression of activator probe, which includes the photolyase homology region of CRY2 (CRY2PHR) and the transcriptional activator domain.
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64125GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.
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64126pNeurog2-Luciferase reporterPhotoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.
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64127Tet-inducible Luciferase reporterPhotoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.
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64128Tet-inducible mCherry reporterPhotoactivatable transcription system. mCherry reporter under the control of a tet-inducible promoter
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64129sgRNA1_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.
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64130sgRNA2_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.
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64131sgRNA3_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette.
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64132sgRNA4_ASCL1Photoactivatable transcription system. Lentiviral expression of ASCL1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette.
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64136sgRNA1_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette.
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64137sgRNA2_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette.
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64138sgRNA3_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette
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64139sgRNA4_MYOD1Photoactivatable transcription system. Lentiviral expression of MYOD1 sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette
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64140sgRNA1_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette
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64142sgRNA2_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette
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64151sgRNA3_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette
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64152sgRNA4_IL1RNPhotoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette
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64153sgRNA1_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette
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64154sgRNA2_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette
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64155sgRNA3_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette
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64156sgRNA4_NANOGPhotoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette
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64157sgRNA1_GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
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64158sgRNA2_GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
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64159sgRNA3_GAL4UAS-Luciferase reporterPhotoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette
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64160sgRNA1_pNeurog2-Luciferase reporterPhotoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.
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64161sgRNA1_Tet-inducible Luciferase reporterPhotoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.
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