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Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells.
Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
64215pAAVS1-TLR targeting vectorTargeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct
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64216pU6-sgRosa26-1_CBh-Cas9-T2A-BFPExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptide
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64217pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)Expression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2A
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64218pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1BExpression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A
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64219pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1BExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A
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64220pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E4orf6Expression vector for sgRNA and Cas9 linked via T2A to BFP linked to the Ad4 E4orf6 gene via P2A
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64221pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E1BExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry and to Ad4 E1B55K via P2A
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64222pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A
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64322pTLR repair vectorVector for repair of the traffic light reporter (TLR), providing the venus coding region
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64323pU6-(BbsI)_CBh-Cas9-T2A-BFPExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide
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64324pU6-(BbsI)_CBh-Cas9-T2A-mCherryExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptide
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