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pAAVS1-TLR targeting vector
(Plasmid #64215)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64215 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAVS1 SA-2A-puro-pA donor
  • Backbone manufacturer
    Jaenisch lab (Addgene plasmid # 22075)
  • Modifications to backbone
    A CAG-Venus+1-P2A+3-tagRFP+3 cassette was cloned into SalI & NotI sites of AAVS1-SA-2A-Puro targeting vector (Addgene plasmid# 22075). The defective Venus (codons 117–152 replaced by a 52-bp segment derived from mouse Rosa26 locus) and a 56-bp segment from the mouse Rab38 gene were linked via T2A peptide to TagRFP in a 2-bp shifted reading frame (+3).
  • Vector type
    Human targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    traffic light reporter (TLR)
  • Alt name
    AAVS1 targeting vector
  • Alt name
    pAAVS1-TLR donor
  • Species
    H. sapiens (human)
  • Mutation
    see publication for details
  • GenBank ID
    S51329.1
  • Entrez Gene
    AAVS1 (a.k.a. AAV)
  • Promoter CAG

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVS1-TLR targeting vector was a gift from Ralf Kuehn (Addgene plasmid # 64215 ; http://n2t.net/addgene:64215 ; RRID:Addgene_64215)
  • For your References section:

    Increasing the efficiency of homology-directed repair for CRISPR-Cas9-induced precise gene editing in mammalian cells. Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R. Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198. 10.1038/nbt.3198 PubMed 25803306