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Doudna Lab CRISPR Plasmids Available from Addgene

Assorted Cas9 nucleases from different bacteria as well as a mammalian codon-optimized Cas9 expression plasmid.

These plasmids are described in:

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. Science . 2012 Jun 28. PUBMED.

Sequence- and structure-specific RNA processing by a CRISPR endonuclease. Haurwitz RE, Jinek M, Wiedenheft B, Zhou K, Doudna JA. Science . 2010 Sep 10;329(5997):1355-8. PUBMED

RNA-programmed genome editing in human cells. Jinek M, East A, Cheng A, Lin S, Ma E, Doudna JA. eLife . 2013 Jan 29;2:e00471. PUBMED

Individual plasmids can be ordered via the links below:

ID Plasmid
39312 pMJ806r : Streptococcus pyogenes Cas9. Add to Cart
39313 pMJ823 : Listeria innocua Cas9. Add to Cart
39314 pMJ824 : Streptococcus thermophilus Cas9. Add to Cart
39315 pMJ825 : Streptococcus pyogenes Cas9 D10A mutation. Add to Cart
39316 pMJ826 : Streptococcus pyogenes Cas9 H840A. Add to Cart
39317 pMJ839 : Neisseria meningitidis Cas9. Add to Cart
39318 pMJ841 : Streptococcus pyogenes Cas9 D10A, H840A. Add to Cart
41091 pHMGWA-Pa14Csy4 : Pseudomonas aeruginosa Csy4 WT. Add to Cart
41092 pHMGWA-Pa14Csy4H29A : Pseudomonas aeruginosa Csy4 H29A. Add to Cart
42234 pMJ920 : Human codon-optimized Cas9 expression plasmid. Add to Cart