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Mouse CRISPR Inhibition Pooled Library (Dolomiti)
(Pooled Library #1000000131, #104090, #104091)

  • Purpose

    This mouse CRISPR inhibition library (Dolomiti) is in backbone pXPR_050 (VP64). This Dolomiti library inhibits over 22,000 mouse genes and is used for genome-wide inhibition screening.

    Each gene inhibited by this library is targeted by 3-6 gRNAs. These gRNAs are split among the two pools: Set A (Cat# 104090) contains gRNAs 1-3, Set B (Cat# 104091) contains gRNAs 4-6. Each pool has a unique set of gRNAs, but both pools are designed to target the same genes. The slight discrepancy in number of genes targeted between Set A and Set B is because not all genes had 6 gRNAs.

    Depositor comments: Primers for the lentiGuide backbone can be used with this library in the Sequencing Protocol.

  • Vector Backbone

Ordering

Mouse CRISPRi sgRNA library Dolomiti, Set A & B (entire library)
A dCas9 inhibition plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lenti KRAB-dCas9 (Addgene #96918).
Pooled Library Set
Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000131 Two tubes, each with a half-library (Set A and B) in backbone XPR_050
  • #104090 (Set A)
  • #104091 (Set B)
1 $500 Add to Cart
Available to Academic and Nonprofits Only

Mouse CRISPRi sgRNA library Dolomiti, Individual items
A dCas9 inhibition plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lenti KRAB-dCas9 (Addgene #96918).
Individual Items
Item Catalog # Description Quantity Price (USD)
Pooled Library 104090 Set A, one tube, contains Mouse CRISPRi sgRNA library Dolomiti 1 $350 Add to Cart
Pooled Library 104091 Set B, one tube, contains Mouse CRISPRi sgRNA library Dolomiti 1 $350 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Mouse
  • Genes targeted
    22,311 (Set A), 22,247 (Set B)
  • gRNAs
    67,366 (Set A), 67,194 (Set B)
  • Controls
    500 unique non-targeting controls are in each of the two half-libraries (Set A and Set B)
  • Lentiviral Generation
    3rd

Library Shipping

These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

Pooled libraries #104090 and #104091 will be delivered as a single tube containing a pooled half-library with 3 gRNAs per gene.

Pooled library #1000000131 will be delivered as two tubes, each containing a pooled half-library with 3 gRNAs per gene (for a total of up to 6 unique gRNAs per gene).

  • Volume
    ∼20µL
  • Concentration
    50ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Dolomiti CRISPR inhibition pooled library set A was a gift from David Root and John Doench (Addgene #104090).
    or
    Mouse Dolomiti CRISPR inhibition pooled library set B was a gift from David Root and John Doench (Addgene #104091).
  • For your References section:

    Up, down, and out: next generation libraries for genome-wide CRISPRa, CRISPRi, and CRISPR-Cas9 knockout genetic screens. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. bioRxiv 356626. doi: 10.1101/356626. bioRxiv 356626