Mouse CRISPR Inhibition Pooled Library (Dolomiti)
(Pooled Library #1000000131, #104090, #104091)
This mouse CRISPR inhibition library (Dolomiti) is in backbone pXPR_050. This Dolomiti library inhibits over 22,000 mouse genes and is used for genome-wide inhibition screening.
Each gene inhibited by this library is targeted by 3-6 gRNAs. These gRNAs are split among the two pools: Set A (Cat# 104090) contains gRNAs 1-3, Set B (Cat# 104091) contains gRNAs 4-6. Each pool has a unique set of gRNAs, but both pools are designed to target the same genes. The slight discrepancy in number of genes targeted between Set A and Set B is because not all genes had 6 gRNAs.
Depositor comments: Primers for the lentiGuide backbone can be used with this library in the Sequencing Protocol.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000131||Two tubes, each with a half-library (Set A and B) in backbone XPR_050†
||1||$500||Add to Cart|
|Pooled Library||104090||Set A, one tube, contains Mouse CRISPRi sgRNA library Dolomiti†||1||$300||Add to Cart|
|Pooled Library||104091||Set B, one tube, contains Mouse CRISPRi sgRNA library Dolomiti†||1||$300||Add to Cart|
Genes targeted22,311 (Set A), 22,247 (Set B)
gRNAs67,366 (Set A), 67,194 (Set B)
Controls500 unique non-targeting controls are in each of the two half-libraries (Set A and Set B)
These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. A tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.
Pooled libraries #104090 and #104091 will be delivered as a single tube containing a pooled half-library with 3 gRNAs per gene.
Pooled library #1000000131 will be delivered as two tubes, each containing a pooled half-library with 3 gRNAs per gene (for a total of up to 6 unique gRNAs per gene).
Terms and Licenses
Please visit https://www.biorxiv.org/content/early/2018/06/27/356626 for bioRxiv preprint.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Mouse Dolomiti CRISPR inhibition pooled library set A was a gift from David Root and John Doench (Addgene #104090).
Mouse Dolomiti CRISPR inhibition pooled library set B was a gift from David Root and John Doench (Addgene #104091).
For your References section:Optimized libraries for CRISPR-Cas9 genetic screens with multiple modalities. Sanson KR, Hanna RE, Hegde M, Donovan KF, Strand C, Sullender ME, Vaimberg EW, Goodale A, Root DE, Piccioni F, Doench JG. Nat Commun. 2018 Dec 21;9(1):5416. doi: 10.1038/s41467-018-07901-8. PubMed 30575746