Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Superti-Furga Lab Human SLC Knockout Library
(Pooled Library #132552)

  • Purpose

    The CRISPR knockout library targets the human solute carrier family of proteins. A set of additional 20 essential genes is also targeted as a control set.

  • Vector Backbone

    lentiCRISPR v2 - Expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 132552 gRNA pooled library 1 $350 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted

    390 SLCs
    20 essential genes
    7 pseudogenes

  • gRNAs
    2,609
  • Controls
    120 non-targeting sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

Superti-Furga Lab Human CRISPR SLC Knockout Library
  • Superti-Furga Lab Human CRISPR SLC Knockout Library

The library results can be analyzed with several packages, including the MAGeCK algorithms or R/DESeq.

The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human SLC Knockout Library was a gift from Giulio Superti-Furga (Addgene #132552)
  • For your References section:

    A widespread role for SLC transmembrane transporters in resistance to cytotoxic drugs. Girardi E, Cesar-Razquin A, Lindinger S, Papakostas K, Konecka J, Hemmerich J, Kickinger S, Kartnig F, Gurtl B, Klavins K, Sedlyarov V, Ingles-Prieto A, Fiume G, Koren A, Lardeau CH, Kumaran Kandasamy R, Kubicek S, Ecker GF, Superti-Furga G. Nat Chem Biol. 2020 Apr;16(4):469-478. doi: 10.1038/s41589-020-0483-3. Epub 2020 Mar 9. PubMed 32152546