Liu Human CRISPR Knockout Library
(Pooled library #1000000132)
This lentiviral genome-scale CRISPR library targets 18,436 human genes with 185,634 guide RNAs. The library is divided into 2 parts (h1 and h2). The h1 and h2 libraries contain in total 10 gRNAs per gene (5 gRNAs in each half library).
gRNA design for this library is optimized to (1) maximize on-target cleavage effects using the SSC algorithm developed by the Liu Lab ( Xu et al. Genome Research 2015), (2) minimize potential off-target effects, and (3) ensure the fidelity of their sequence with corresponding target loci and avoid regions with possible genomic variations.
lentiCRISPRv2 - does express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000132||gRNA pooled library||1||$500||Add to Cart|
gRNAs92,817 in each half library
This library is delivered as suspended DNA in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaws.
The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.
Addgene recommends using electrocompetent cells for amplification to ensure 50X coverage.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human lentiviral CRISPR library was a gift from Xiaole Shirley Liu (Addgene #1000000132)