Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is open for ordering and depositing; find up-to-date details here. To learn more about how we are supporting COVID-19 research and to find related plasmids, check out our COVID-19 and Coronavirus Plasmids & Resources page.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Lu SPECS Library
(Pooled Library #127842)

  • Purpose

    The Synthetic Promoters with Enhanced Cell-State Specificity (SPECS) library enables high-throughput screening and promoter identification.

    Library constructs are composed of tandem repeats of transcription factor binding sites upstream of an adenovirus minimal promoter controlling expression of mKate2.

  • Vector Backbone

    FUW

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 127842 Promoter pooled library 1 $400 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Synthetic promoters
    6,107 unique motifs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

Lu Lab SPECS Library
  • Lu Lab SPECS Library

The depositing lab has shared their analysis pipeline through Github: SPECS.

The Lu Lab has provided two accessory plasmids to accompany the SPECS Library:
Plasmid 128569: Accessory plasmid 1- Mix with the library during lentiviral production
Plasmid 128571: Accessory plasmid 2- Used to clone identified synthetic promoters

NOTE: Lentiviral vectors can recombine during library amplification, resulting in an ~1-1.7kb band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the PuroR gene and will not be selected during screening. If this ~1.7.kb band is observed after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SPECS Library was a gift from Timothy Lu (Addgene #127842)
  • For your References section:

    A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS). Wu MR, Nissim L, Stupp D, Pery E, Binder-Nissim A, Weisinger K, Enghuus C, Palacios SR, Humphrey M, Zhang Z, Maria Novoa E, Kellis M, Weiss R, Rabkin SD, Tabach Y, Lu TK. Nat Commun. 2019 Jun 28;10(1):2880. doi: 10.1038/s41467-019-10912-8. PubMed 31253799