Lu SPECS Library
(Pooled Library #127842)
The Synthetic Promoters with Enhanced Cell-State Specificity (SPECS) library enables high-throughput screening and promoter identification.
Library constructs are composed of tandem repeats of transcription factor binding sites upstream of an adenovirus minimal promoter controlling expression of mKate2.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||127842||Promoter pooled library||1||$400||Add to Cart|
Synthetic promoters6,107 unique motifs
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
The depositing lab has shared their analysis pipeline through Github: SPECS.
The Lu Lab has provided two accessory plasmids to accompany the SPECS Library:
Plasmid 128569: Accessory plasmid 1- Mix with the library during lentiviral production
Plasmid 128571: Accessory plasmid 2- Used to clone identified synthetic promoters
NOTE: Lentiviral vectors can recombine during library amplification, resulting in an ~1-1.7kb band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the PuroR gene and will not be selected during screening. If this ~1.7.kb band is observed after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SPECS Library was a gift from Timothy Lu (Addgene #127842)
For your References section:A high-throughput screening and computation platform for identifying synthetic promoters with enhanced cell-state specificity (SPECS). Wu MR, Nissim L, Stupp D, Pery E, Binder-Nissim A, Weisinger K, Enghuus C, Palacios SR, Humphrey M, Zhang Z, Maria Novoa E, Kellis M, Weiss R, Rabkin SD, Tabach Y, Lu TK. Nat Commun. 2019 Jun 28;10(1):2880. doi: 10.1038/s41467-019-10912-8. PubMed 31253799