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FUW
(Plasmid #14882)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14882 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    HR'CS-G
  • Backbone manufacturer
    I. Verma, Salk
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    flap-Ub promoter-WRE

Cloning Information

Resource Information

Depositor Comments

Plasmid pFUW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap
sequence PCR-amplified
from the HIV NLA4.3 genome, the human polyubiquitin
promoter-C (gift of L. Thiel, Amgen), and the WRE (woodchuck
hepatitis virus posttranscriptional
regulatory element) (gift of D.
Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging
vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.

Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI HpaI XhoI AscI EcoRI ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI. Unique sites for subcloning in the MCS are: BamHI, HpaI, AscI, and EcoRI.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUW was a gift from David Baltimore (Addgene plasmid # 14882 ; http://n2t.net/addgene:14882 ; RRID:Addgene_14882)
  • For your References section:

    Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D. Science. 2002 Feb 1. 295(5556):868-72. 10.1126/science.1067081 PubMed 11786607