|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14882||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerI. Verma, Salk
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameflap-Ub promoter-WRE
- Cloning method Restriction Enzyme
- 5′ sequencing primer na (Common Sequencing Primers)
Plasmid pFUW was constructed by inserting the following into the multicloning site of HR'CS-G: HIV-1 flap
from the HIV NLA4.3 genome, the human polyubiquitin
promoter-C (gift of L. Thiel, Amgen), and the WRE (woodchuck
hepatitis virus posttranscriptional
regulatory element) (gift of D.
Trono, University of Geneva). Lentiviruses can be produced by cotransfecting the HIV-1 packaging
vector Delta8.9 and the VSVG envelope glycoprotein into 293 fibroblasts.
Order of elements: CMV LTR PstI flap PacI Ubiquitin promoter SpeI HindIII PstI SalI XbaI BamHI HpaI XhoI AscI EcoRI ClaI WRE ClaI SalI XhoI KpnI 3'LTR ApaI PmeI. Unique sites for subcloning in the MCS are: BamHI, HpaI, AscI, and EcoRI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FUW was a gift from David Baltimore (Addgene plasmid # 14882 ; http://n2t.net/addgene:14882 ; RRID:Addgene_14882)
For your References section:Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D. Science. 2002 Feb 1. 295(5556):868-72. 10.1126/science.1067081 PubMed 11786607