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Neelamegham Human GlycoGene CRISPR Library
(Pooled Library #140961)

  • Purpose

    The GlycoGene CRISPR lentiviral library targets human genes involved in cellular glycosylation. These include glycosyltransferase genes involved in biosynthesis of several different types of glycoconjugates, including: N-glycans, O-glycans, glycolipids, glycosaminoglycans, GPI-anchored carbohydrates, O-fucose, O-GlcNAc, O-Glucose, O-Mannose, and EGF domain specific O-linked GlcNAcTs (EOGTs).

    This library also targets proteins involved in intracellular protein transport, sulfotransferases, enzymes involved in nucleotide-sugar biosynthesis, nucleotide-sugar transporters and glycosidases.

  • Vector Backbone

    SiC-V2-Cas9G7 - does not express Cas9

    This vector was modified as described in the associated publication. The mCerulean gene has been replaced with TagBFP. For library construction the sgRNA cloning region of lentiCRISPR V2 was cloned downstream of the U6 promoter.

    The full backbone vector sequence can be found in the Resource Information section.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 140961 gRNA pooled library 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. The library can be used in conjunction with any SpCas9 expressing plasmid, such as lentiCas9-Blast (Addgene #52962) or SiC-V1 (Addgene #133041). Alternatively, cell lines expressing SpCas9 or cells electroporated with Cas9 protein can be used for screening.

Library Details

  • Species
    Human
  • Genes targeted
    347
  • gRNAs
    3637
  • Controls
    Non-targeting gRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼10µL
  • Concentration
    10ng/µL

Depositor Comments

The depositing lab has created a web resource to facilitate use of the GlycoGene CRISPR Library: GlycoGene CRISPR Library Viewer

They recommend using MAGeCK/MAGeCK-VISPR algorithms for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human GlycoGene CRISPR library was a gift from Sriram Neelamegham (Addgene #140961)
  • For your References section:

    A GlycoGene CRISPR-Cas9 lentiviral library to study lectin binding and human glycan biosynthesis pathways. Zhu Y, Groth T, Kelkar A, Zhou Y, Neelamegham S. Glycobiology. 2020 Aug 8. pii: 5889976. doi: 10.1093/glycob/cwaa074. PubMed 32776087