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Human mTORC1 Focused Library
(Pooled Library #164084)

  • Purpose

    This lentiviral CRISPR library targets human genes required for mTORC1 activation and inhibition with 10 sgRNAs per gene.

  • Vector Backbone

    lentiCRISPR v1- expresses Cas9

    Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages recipient scientists to use the updated lentiCRISPR v2, which can be requested separately.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 164084 mTORC1 Focused Library 1 $350 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    712
  • gRNAs
    7,501
  • Controls

    246 targeting control sgRNA
    129 intergenic control sgRNA
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

  • Sabatini Lab mTORC1 Focused Library

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human mTORC1 Focused Library was a gift from David Sabatini (Addgene #164084)
  • For your References section:

    Genome-wide CRISPR screens reveal multitiered mechanisms through which mTORC1 senses mitochondrial dysfunction. Condon KJ, Orozco Jm, Adelmann CH, Spinelli JB, van der Helm PW, Roberts JM, Kunchok T, Sabatini DM. bioRxiv 2020.10.22.351361. bioRxiv 10.22.351361