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Vakoc Human Kinase Domain-Focused CRISPR Knockout Library
(Pooled Library #117725)

  • Purpose
    This lentiviral CRISPR knockout library contains gRNAs that target only the kinase domain of protein kinases. Targeting the functional domain of proteins enhances the severity of the negative selection screen, as reported previously by the Vakoc lab ( Shi et al. Nat Biotechnol 2015).
  • Vector Backbone

    LRG2.1 - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 117725 gRNA pooled library 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with LentiV_Cas9_puro (Addgene #108100) or otherwise with cell lines expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    482 kinase genes (496 domains)
  • gRNAs
    3,051
  • Controls
    36 positive, 51 negative
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

Lentiviral vectors can recombine during library amplification, resulting in a lower molecular weight band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the EGFP gene and will not be selected during screening. If a lower molecular weight band is observed after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Kinase Domain-Focused CRISPR Knockout library was a gift from Christopher Vakoc (Addgene #117725)
  • For your References section:

    LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia. Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, Klingbeil O, El Demerdash O, Shi J, Vakoc CR. Mol Cell. 2018 Mar 15;69(6):1017-1027.e6. doi: 10.1016/j.molcel.2018.02.011. PubMed 29526696