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CRiNCL - Human CRISPRi Non-coding Libraries
(Pooled Libraries #86538, #86539, #86540, #86541, #86542, #86543, #86544, #86545, #86546, #86547, #86548, #86549, #86550)

  • Purpose

    These CRISPRi subpooled libraries (10 gRNAs/TSS) are designed to target long non-coding RNA (lncRNA)-expressing genes rather than coding genes in order to discover non-coding genes with cellular functions.

  • Vector Backbone

    pCRISPRia-v2 - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 86538 CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines 1 $600 Add to Cart
Pooled Library 86539 CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines 1 $600 Add to Cart
Pooled Library 86540 CRISPRi non-coding library (CRiNCL) - Unique to iPSC 1 $600 Add to Cart
Pooled Library 86541 CRISPRi non-coding library (CRiNCL) - Unique to HFF 1 $600 Add to Cart
Pooled Library 86542 CRISPRi non-coding library (CRiNCL) - Unique to U87 1 $600 Add to Cart
Pooled Library 86543 CRISPRi non-coding library (CRiNCL) - Unique to HEK293T 1 $600 Add to Cart
Pooled Library 86544 CRISPRi non-coding library (CRiNCL) - Unique to K562 1 $600 Add to Cart
Pooled Library 86545 CRISPRi non-coding library (CRiNCL) - Unique to HeLa 1 $600 Add to Cart
Pooled Library 86546 CRISPRi non-coding library (CRiNCL) - Unique to MCF7 1 $600 Add to Cart
Pooled Library 86547 CRISPRi non-coding library (CRiNCL) - U87 & HEK293T 1 $600 Add to Cart
Pooled Library 86548 CRISPRi non-coding library (CRiNCL) - K562, HeLa, & MCF7 1 $600 Add to Cart
Pooled Library 86549 CRISPRi non-coding library (CRiNCL) - K562 & HeLa 1 $600 Add to Cart
Pooled Library 86550 CRISPRi non-coding library (CRiNCL) - iPSC & HFF 1 $600 Add to Cart
This material is available to academics and nonprofits only.

  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB-BFP (#46911) or otherwise used with cell lines already expressing inhibitory dCas9.

  • If you would like to receive all thirteen sublibraries in a single order, please email [email protected] for pricing.

Library Details

Pooled Library Genes Targeted gRNAs Control gRNAs
86538 1329 13,548 258
86539 675 6903 153
86540 2965 29,976 326
86541 4103 41,511 481
86542 2307 23,317 247
86543 2253 22,869 339
86544 2831 28,501 191
86545 2337 23,693 323
86546 2913 29,617 487
86547 1443 14,694 264
86548 875 8944 194
86549 1051 10,794 284
86550 1269 12,950 260
  • Species
    Human
  • Lentiviral Generation
    3rd

Library Shipping

Each library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

  • Volume
    ~20 µL
  • Concentration
    50 ng/µL

Depositor Comments

The data analysis pipeline for analyzing sequencing data is available on GitHub.

schematic of crincl sublibraries

Schematic of CRiNCL sublibraries

How to cite this pooled library (Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene # )
  • For your References section:

    CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA. Science. 2017 Jan 06. Vol. 355, Issue 6320. pii: aah7111. PubMed 27980086