Weissman Human CRISPRi Titration
(Pooled Libraries #136478, #136479)
These libraries contain ~5,000 series of systematically attenuated CRISPRi gRNAs targeting ~2,500 genes involved in central biological processes. The activity of gRNAs within each series vary from fully functional to inactive. When paired with CRISPRi, each series enables titration of a targeted gene from normal expression level to completely knocked down.
The large-scale library (Addgene #136478) contains two series of gRNA per gene with 23-24 gRNA per series.
The compact library (Addgene #136479) contains two series of gRNA per gene with 1-9 gRNA per series.
pCRISPRia-v2 - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||136478||Large-scale gRNA Pooled Library†||1||$500||Add to Cart|
|Pooled Library||136479||Compact gRNA Pooled Library†||1||$350||Add to Cart|
Genes targeted2,449 (Large-scale), 2,405 (Compact)
gRNAs119,510 (Large-scale), 25,518 (Compact)
Controls2,500 non-targeting gRNAs (Large-scale), 1,202 non-targeting gRNAs (Compact)
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Weissman Human CRISPRi Titration library was a gift from Jonathan Weissman (Addgene #)
For your References section:Titrating Gene Expression Using Libraries of Systematically Attenuated CRISPR Guide RNAs. Jost M, Santos DA, Saunders RA, Horlbeck MA, Hawkins JS, Scaria SM, Norman TM, Hussmann JA, Liem CR, Gross CA, Weissman JS. Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0387-5. doi: 10.1038/s41587-019-0387-5. PubMed 31932729