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Weissman Human CRISPRi Titration
(Pooled Libraries #136478, #136479)

  • Purpose

    These libraries contain ~5,000 series of systematically attenuated CRISPRi gRNAs targeting ~2,500 genes involved in central biological processes. The activity of gRNAs within each series vary from fully functional to inactive. When paired with CRISPRi, each series enables titration of a targeted gene from normal expression level to completely knocked down.

    The large-scale library (Addgene #136478) contains two series of gRNA per gene with 23-24 gRNA per series.

    The compact library (Addgene #136479) contains two series of gRNA per gene with 1-9 gRNA per series.

  • Vector Backbone

    pCRISPRia-v2 - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 136478 Large-scale gRNA Pooled Library 1 $500 Add to Cart
Pooled Library 136479 Compact gRNA Pooled Library 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911), pMH0001 (Addgene #85969), or any other plasmids expressing dCas9-KRAB.

Library Details

  • Species
    Human
  • Genes targeted
    2,449 (Large-scale), 2,405 (Compact)
  • gRNAs
    119,510 (Large-scale), 25,518 (Compact)
  • Controls
    2,500 non-targeting gRNAs (Large-scale), 1,202 non-targeting gRNAs (Compact)
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume

    ∼40µL (Large-scale)
    ~20µL (Compact)
  • Concentration

    50ng/µL (Large-scale)
    25ng/µL (Compact)

Depositor Comments

Weissman Lab Mismatched sgRNA Titration Libraries
  • Weissman Lab Mismatched sgRNA CRISPRi Titration Libraries

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Weissman Human CRISPRi Titration library was a gift from Jonathan Weissman (Addgene #)
  • For your References section:

    Titrating Gene Expression Using Libraries of Systematically Attenuated CRISPR Guide RNAs. Jost M, Santos DA, Saunders RA, Horlbeck MA, Hawkins JS, Scaria SM, Norman TM, Hussmann JA, Liem CR, Gross CA, Weissman JS. Nat Biotechnol. 2020 Jan 13. pii: 10.1038/s41587-019-0387-5. doi: 10.1038/s41587-019-0387-5. PubMed 31932729