Showing: 181 - 200 of 563 results
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Plasmid#48117PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
UseTagsExpressionBacterialMutationPromoterPxylAAvailabilityAcademic Institutions and Nonprofits only -
TM1442-pET-21a
Plasmid#12650DepositorInsertPutative anti-sigma factor antagonist (TM1442 T. maritima)
UseTagsExpressionBacterialMutationN/APromoterAvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-479.wt
Plasmid#13527DepositorInsertB. subtilis DEAD-box helicase YxiN
UseTagsintein-chitin binding domainExpressionBacterialMutationnonePromoterAvailabilityAcademic Institutions and Nonprofits only -
pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3AvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-I
Plasmid#53437Purposexylose-dependent expression of the type-I agrC and agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailabilityAcademic Institutions and Nonprofits only -
pXylA-agrCA-IV
Plasmid#53438Purposexylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureusDepositorInsertagrCA locus
UseSynthetic BiologyTagsnoneExpressionBacterialMutation"MVQTS" sequence added to the N-terminu…PromoterPxylAAvailabilityAcademic Institutions and Nonprofits only -
pCG-D-mSpyCatcher-His6-E
Plasmid#87376PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsHis6 tagExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterD-mannoseAvailabilityAcademic Institutions and Nonprofits only -
pGK12
Plasmid#186342PurposeShuttle vector, pWVO1 origin of replication (from L. lactis sbsp. cremoris Wg2). Replicates in both Gram-Positive and Gram-Negative bacteria. Chloramphenicol- and Erythromycin resistance marker.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMZ1001
Plasmid#204398PurposeA pC-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRpa0002AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_13
Plasmid#60584Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_13
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.wt
Plasmid#13528DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479PromoterAvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a404-479.wt
Plasmid#13529DepositorInsertB. subtilis DEAD-box helicase YxiN specific RNA binding domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdelete amino acid residues 1-403PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAPB02
Plasmid#65628PurposeA transformation-associated recombination (TAR) based genomic capture and expression vector for antibiotic production in B. subtilisDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pT7-agrBD-I
Plasmid#53439PurposeT7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220DepositorInsertagrBD locus
UseSynthetic BiologyTagsV5 epitope, 6x HIS (not expressed on agrB or agrD…ExpressionBacterialMutationnonePromoterT7-lacAvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
UseTagsExpressionBacterialMutationA309GPromoterAvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7AvailabilityAcademic Institutions and Nonprofits only
Showing: 181 - 200 of 563 results