Addgene

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

New search

Keep filters

3,551 results

Showing: 101 - 120 of 3551 results

Results per page:

pGEX-2T-TRR-C421-G2304D

Plasmid

#182845

Purpose

mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Use

Tags

GST

Expression

Bacterial

Mutation

mutation G2304D (GGC/GAC)

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T-TRR-C421-Y2404F

Plasmid

#182846

Purpose

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Article

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431

Use

Tags

Expression

Bacterial

Mutation

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1056D

Plasmid

#183132

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii bTub, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1056E

Plasmid

#183133

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii dsx, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[dsx]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1056F

Plasmid

#183134

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii tra, Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[tra]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1056H

Plasmid

#183135

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1056J

Plasmid

#183136

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1056K

Plasmid

#183137

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
1161F

Plasmid

#183138

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pALuWBB

Plasmid

#183202

Purpose

dsRed marked transgene for Crispr co-conversion with w+ gRNA and drop in cloning sites for 2 more gRNA

Depositor

Mitch McVey

Insert

w+ gRNA and scaffold followed by 2 gRNA with BbsI and BsaI cloning sites

Use

CRISPR; Recombines at attp landing site in fly ge…

Tags

Expression

Insect

Mutation

Promoter

U6-3

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-SU(Z)12(624-900)-H6

Plasmid

#183772

Purpose

Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

Insert

SU(Z)12 residues 624-900

Use

Tags

His tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-UTX(420-633)-H6

Plasmid

#183773

Purpose

Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

UTX

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2T-CBP(1603-2678)

Plasmid

#183774

Purpose

Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

nej

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11-PC-H6

Plasmid

#183777

Purpose

Express PC-H6 in E. coli. Purified PC-H6 was used for for generating antibodies in rabbits and for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11-PC(75-228)-H6

Plasmid

#183779

Purpose

Express PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb

Use

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PC(1-86)-H6

Plasmid

#183780

Purpose

Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb

Use

Tags

6 His Tag at the C-terminus

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pET11-PC(75-390)-H6

Plasmid

#183781

Purpose

Express PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assays

Depositor

Peter Harte

Article

Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

Insert

Polycomb (w Fly)

Use

Tags

6 His tag at the C-terminus

Expression

Bacterial

Mutation

6 His tag at the C-terminus

Promoter

T7

Availability

Academic Institutions and Nonprofits only

Enlarge
pUASp-Egl-Trbo

Plasmid

#179993

Purpose

Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

Egl-Trbo

Use

Tags

TurboID

Expression

Insect

Mutation

Promoter

UAS-Gal4

Availability

Academic Institutions and Nonprofits only

Enlarge
puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge