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Showing: 21 - 40 of 69 results
  1. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ...considered a linker (figure 1). In fact, for several FRET biosensors (yellow cameleon 3.60, EPAC, and Galphai...fluorescent protein can be truncated to increase the FRET efficiency. Creating fusions at the N- and C- termini...Klarenbeek, Jeffrey, et al. "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness,...PubMed PMID: 30886412. Mastop, Marieke, et al. "A FRET-based biosensor for measuring Gα13 activation in.... van Unen, Jakobus, et al. "A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and...
  2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    ...more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities: Tracking intracellular...Central PMCID: PMC3984138. Microscopy U. Basics of FRET Microscopy. Additional Resources on the Addgene...Fluorescent Protein Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute...Science Plasmid Collection Check Out Plasmids for FRET Check Out the Fluorescent Protein Guide ...
  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...cause an effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy...excites the fluorescence of another FP (e.g. YFP). FRET only occurs when the distance between the two FPs...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins interact...
  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...domain being tested. Find FRET plasmids here. Split EGFP: An alternative to FRET, split EGFP has also been... uses of GFP. Förster resonance energy transfer (FRET): This is used to study the interactions between...concentrations and pH, using a range of strategies such as FRET, calmodulin, and others. Review Addgene's collection...
  5. Bacterial Expression Systems

    Type
    Collection
    ...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression...found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible expression...fluorescent protein variant (CyPet) optimized for FRET measurements with a yellow fluorescent protein variant...fluorescent protein variant (YPet) optimized for FRET measurements with a cyan fluorescent protein variant... and containing 3-AT. FLIParaF.Ec-250n 18855 N/A FRET between CFP and YFP Wolf Frommer Reporter for arabinose...bacteria. The presence of arabinose enhances the FRET signal from the CFP and YFP fluorophores linked ...
  6. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post
    ... tips from experts on FRET techniques. By combining standard donor/acceptor FRET know-how with aptamers...Aptabeacons Fluorescence resonance energy transfer (FRET) technology has been studied and used in assays ...Additional Resources on the Addgene Blog Tips for Using FRET in Your Experiments Learn more about GFP Controlling...
  7. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...
  8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...cells are then grown in biotin-containing media. FRET is another example of a protein fragmentation complementation...used to study binary protein interactions. However, FRET generally only tells you if two proteins interact...Find Plasmids from the Roux Lab Find Plasmids for FRET ...
  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...This technique is very similar to the widely used FRET, but it eliminates the need for an excitation light...Check Out All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein...
  10. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...using fluorescently tagged ligands or intermolecular FRET for ligand-receptor interactions, require specific...tagging. In Fluorescence Resonance Energy Transfer (FRET) applications, the dyes need to have compatible ...profiles is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594...
  11. Addgene's eBook Collection

    Type
    Guide
    ...for choosing a fluorescent protein; information on FRET, biosensors, and monitoring protein localization...
Showing: 21 - 40 of 69 results