pMT3-RhoGC E254D
(Plasmid #100033)

• Purpose: This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.
• Depositing Lab: Daniel Oprian
• Publication: Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-10389. doi: 10.1074/jbc.M117.789636. Epub 2017 May 4.

Backbone

• Vector backbone: pMT3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RhoGC E254D
• Species: B. emersonii
• Mutation: E254D
• Promoter: Adenovirus major late promoter
• Tag / Fusion Protein:
  • 1D4 (TETSQVAPA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: TGC CTT TGA GGG TGG CCG
• 3′ sequencing primer: GCC GTC AAG TTT GGC GCG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alanine insertion at the second position of the RhoGC amino acid sequence does not affect function. Mutation numbering is relative to the wild type reference sequence [KF309499].