Expression, purification, and spectral tuning of RhoGC, a retinylidene/guanylyl cyclase fusion protein and optogenetics tool from the aquatic fungus Blastocladiella emersonii.
Trieu MM, Devine EL, Lamarche LB, Ammerman AE, Greco JA, Birge RR, Theobald DL, Oprian DD
J Biol Chem. 2017 Jun 23;292(25):10379-10389. doi: 10.1074/jbc.M117.789636. Epub 2017 May 4. PubMed Article
Trieu MM, Devine EL, Lamarche LB, Ammerman AE, Greco JA, Birge RR, Theobald DL, Oprian DD
J Biol Chem. 2017 Jun 23;292(25):10379-10389. doi: 10.1074/jbc.M117.789636. Epub 2017 May 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 100031 | pMT3-RhoGC | This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein. |
| 100032 | pMT3-RhoAC | This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity. |
| 100033 | pMT3-RhoGC E254D | This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm. |
| 100034 | pMT3-RhoGC D380N | This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm. |
| 100035 | pMT3-RhoGC D380E | This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm. |