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pSN35
(Plasmid #100120)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100120 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLM-vexGFP-OCT4
  • Backbone manufacturer
    Michel Sadelain (Addgene plasmid # 22240)
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    vexGFP-P2A-OCT4 fused to an Engrailed domain (168-281) separated by a linker
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSN35 was a gift from Mark Ptashne (Addgene plasmid # 100120 ; http://n2t.net/addgene:100120 ; RRID:Addgene_100120)
  • For your References section:

    OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Narayan S, Bryant G, Shah S, Berrozpe G, Ptashne M. Cell Rep. 2017 Aug 15;20(7):1585-1596. doi: 10.1016/j.celrep.2017.07.071. 10.1016/j.celrep.2017.07.071 PubMed 28813671