pEV_S
(Plasmid #100266)

• Purpose: pDSW204 carrying genes entABCDE, vibHF and a cos site, for serratiochelin and analogs production in E. coli
• Depositing Lab: Timothy Lu
• Publication: Cleto et al MBio. 2017 Oct 10;8(5). pii: e01474-17. doi: 10.1128/mBio.01474-17.

Backbone

• Vector backbone: pDSW204
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 18830
• Modifications to backbone: Added cos site for plasmid stability with large inserts.
• Vector type: Bacterial Expression, Synthetic Biology ; Expression, pBR322

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: In minimal medium, grow at 30C.
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: entD
• Species: Escherichia coli
• GenBank ID: ID 945194
• Entrez Gene: entD (a.k.a. b0583, ECK0575)

Gene/Insert 2

• Gene/Insert name: entC
• Species: Escherichia coli
• GenBank ID: ID 945511

Gene/Insert 3

• Gene/Insert name: entE
• Species: Escherichia coli
• GenBank ID: ID 947426

Gene/Insert 4

• Gene/Insert name: entB
• Species: Escherichia coli
• GenBank ID: ID 946178

Gene/Insert 5

• Gene/Insert name: entA
• Species: Escherichia coli
• GenBank ID: ID 945284

Gene/Insert 6

• Gene/Insert name: vibH
• Species: Vibrio cholerae
• GenBank ID: ID 2615318

Gene/Insert 7

• Gene/Insert name: vibF
• Species: Vibrio cholerae
• GenBank ID: ID 2614958

Gene/Insert 8

• Gene/Insert name: cos

Resource Information

• Supplemental Documents:
  • pEV_S.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results found an A57E mutation in entE and a Q182R mutation in entA. Plasmid functions as described.