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pSL144
(Plasmid #252945)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252945 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pUC(pGEM) ori_256rep_cat_ampR (modified from pSIP403)
  • Vector type
    Bacterial Expression
  • Selectable markers
    Chloramphenicol resistance (5 ug/mL) in Lactiplantibacillus plantarum

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sfGFP
  • Alt name
    P23_sfgfp
  • Species
    Synthetic
  • Promoter P23

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.24.678173 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSL144 was a gift from Caroline Ajo-Franklin (Addgene plasmid # 252945 ; http://n2t.net/addgene:252945 ; RRID:Addgene_252945)
  • For your References section:

    Synthetic microbial co-cultures for modular bioelectronic sensing in diverse environments. Li S, Zhu D, Saha K, Kundu BB, Sonkusale S, Britton RA, Ajo-Franklin CM. Nat Biotechnol (2026). 10.1038/s41587-026-03075-7