PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100735||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneGTSE1 (a.k.a. B99)
- Promoter CMV
/ Fusion Proteins
- FKBP (N terminal on insert)
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The numbering for this plasmid is based on the longer isoform. The shorter isoform, present in this plasmid, contains amino acids 505 through 586.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:FKBP-GTSE1(638-720)-GFP was a gift from Stephen Royle (Addgene plasmid # 100735 ; http://n2t.net/addgene:100735 ; RRID:Addgene_100735)
For your References section:New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ. J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. 10.1083/jcb.201702188 PubMed 28954824