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New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision.
Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ
J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
100726FKBP-beta2(616-951)-GFPTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100727FKBP-beta2(616-951)-GFP Y215ATo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis
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100728FKBP-beta2(616-951)-GFP ∆CBMTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis
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100729FKBP-beta2(616-951)-GFP DoubleTo express a clathrin "hook" mutant in mammalian cells to hot-wire endocytosis
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100730FKBP-beta2(616-951)-mRuby2To express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100731FKBP-alpha(740-977)-GFPTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)
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100732FKBP-beta1(617-949)-GFPTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100733FKBP-epsin(144-575)-GFPTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100734FKBP-beta3(702-1094)-GFPTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100735FKBP-GTSE1(638-720)-GFPTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100736PDZb-beta2(616-951)-mCherryTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis
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100738CD8-mCherry-FRBTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis
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100739CD8-dCherry-FRBTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)
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100740Myc-CD4-mCherry-HA-FRBTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis
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100741SH4-FRB-mRFPTo express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis
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100742CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis
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100743GST-beta2(616-951)Bacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form
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100744GST-beta2(616-951)Y815ABacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant
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100745GST-beta2(616-951)∆CBMBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant
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100746GST-beta2(616-951)DoubleBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice form, mutant
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100748MBP-CHC(1-1074)-His6Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.
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