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FKBP-beta2(616-951)-GFP Double
(Plasmid #100729)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100729 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AP2B1
  • Alt name
    beta2 adaptin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1008
  • Mutation
    Long isoform, deletion of clathrin box motif and mutation of Y215A
  • GenBank ID
    BC006201 NM_001030006.1
  • Entrez Gene
    AP2B1 (a.k.a. ADTB2, AP105B, AP2-BETA, CLAPB1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FKBP (N terminal on insert)
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The mutation numbering in this plasmid refers to the shorter isoform. The mutation in the longer isoform of AP2B1, which is contained in this plasmid, is Y829A.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FKBP-beta2(616-951)-GFP Double was a gift from Stephen Royle (Addgene plasmid # 100729 ; http://n2t.net/addgene:100729 ; RRID:Addgene_100729)
  • For your References section:

    New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ. J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. 10.1083/jcb.201702188 PubMed 28954824