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MBP-CHC(1-1074)-His6
(Plasmid #100748)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 100748 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CLTC
  • Alt name
    CHC
  • Alt name
    CHC17
  • Alt name
    clathrin heavy chain
  • Species
    H. sapiens (human)
  • Mutation
    Residues 1-1074
  • Entrez Gene
    CLTC (a.k.a. CHC, CHC17, CLH-17, CLTCL2, Hc, MRD56)
  • Tags / Fusion Proteins
    • MBP (N terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site BamHI/BglII (destroyed during cloning)
  • 5′ sequencing primer MBP-F
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MBP-CHC(1-1074)-His6 was a gift from Stephen Royle (Addgene plasmid # 100748 ; http://n2t.net/addgene:100748 ; RRID:Addgene_100748)
  • For your References section:

    New tools for "hot-wiring" clathrin-mediated endocytosis with temporal and spatial precision. Wood LA, Larocque G, Clarke NI, Sarkar S, Royle SJ. J Cell Biol. 2017 Sep 27. pii: jcb.201702188. doi: 10.1083/jcb.201702188. 10.1083/jcb.201702188 PubMed 28954824