GST-TACC3-His6
(Plasmid #69106)

• Purpose: For bacterial expression of recombinant human TACC3 tagged with GST and His.
• Depositing Lab: Stephen Royle
• Publication: Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife.07635.

Backbone

• Vector backbone: pGEX-6p-1
• Backbone size w/o insert (bp): 4997
• Total vector size (bp): 7511
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Transforming Acidic Coiled Coil protein 3
• Alt name: TACC3
• Alt name: ERIC1
• Species: H. sapiens (human)
• Insert Size (bp): 2514
• GenBank ID: NM_006342.2
• Entrez Gene: TACC3 (a.k.a. ERIC-1, ERIC1, Tacc4, maskin)
• Tags / Fusion Proteins:
  • GST (N terminal on backbone)
  • His6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XmaI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Resource Information

• A portion of this plasmid was derived from a plasmid made by: TACC3: IMAGE 6148176

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry