pXJ42-p200 CUX1
(Plasmid #100813)

• Purpose: Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag
• Depositing Lab: Alain Nepveu
• Publication: Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. doi: 10.1074/jbc.M803808200. Epub 2008 Oct 24.

Backbone

• Vector backbone: pXJ42
• Vector type: Mammalian Expression ; Note that this plasmid does not have an SV40 ori

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p200 CUX1
• Species: H. sapiens (human)
• GenBank ID: P39880
• Entrez Gene: CUX1 (a.k.a. CASP, CDP, CDP/Cut, CDP1, COY1, CUTL1, CUX, Clox, Cux/CDP, GDDI, GOLIM6, Nbla10317, p100, p110, p200, p75)
• Tags / Fusion Proteins:
  • Myc (N terminal on insert)
  • HA (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pxj42 p200 CUX1 (1-1505).gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pXJ42 was derived (by another investigator) from pXJ40 (Cell 65:551-568, (1991) by changing the polylinker.
It contains the human CMV promoter and intron 2 from the rabbit beta-globin gene
We used pXJ42 originally because we needed a mammalian vector that did not contain the origin of replication of SV40. In practice, people in the lab found this was the best vector and we continued to use it for other applications.