PurposeAAV expression of ultrasensitive protein calcium sensor from CAG promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100840||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
|AAV1||100840-AAV1||Virus (100 µL at titer ≥ 1×10¹³ vg/mL)|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Alt nameGCaMP3 variant 630
Insert Size (bp)1353
MutationGCaMP3-T302L R303P M378G K379S D380Y T381R S383T R392G
- Promoter CAG
- Cloning method Unknown
- 5′ sequencing primer unknown (Common Sequencing Primers)
This plasmid was previously available as pAAV.CAG.GCaMP6m.WPRE.SV40 (P3080) from the Penn Vector Core. This plasmid was created as part of the GENIE project at Janelia Research Campus.
Information for AAV1 (Catalog # 100840-AAV1) ( Back to top )
Ready-to-use AAV1 particles produced from pAAV.CAG.GCaMP6m.WPRE.SV40 (#100840). In addition to the viral particles, you will also receive purified pAAV.CAG.GCaMP6m.WPRE.SV40 plasmid DNA.CAG-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.
- Volume 100 µL
- Titer ≥ 1×10¹³ vg/mL
- Pricing $350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- Storage Store at -80℃. Thaw just before use and keep on ice.
- Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV1 cap gene
- Buffer PBS + 0.001% Pluronic F-68
- Serotype AAV1
- Purification Iodixanol gradient ultracentrifugation
Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV.CAG.GCaMP6m.WPRE.SV40 was a gift from The Genetically Encoded Neuronal Indicator and Effector Project (GENIE) & Douglas Kim (Addgene plasmid # 100840 ; http://n2t.net/addgene:100840 ; RRID:Addgene_100840)
For viral preps, please replace (Addgene plasmid # 100840) in the above sentence with: (Addgene viral prep # 100840-AAV1)
For your References section:Ultrasensitive fluorescent proteins for imaging neuronal activity. Chen TW, Wardill TJ, Sun Y, Pulver SR, Renninger SL, Baohan A, Schreiter ER, Kerr RA, Orger MB, Jayaraman V, Looger LL, Svoboda K, Kim DS. Nature. 2013 Jul 18;499(7458):295-300. doi: 10.1038/nature12354. 10.1038/nature12354 PubMed 23868258