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pR2Q144c
(Plasmid #101023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101023 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 101023-D50 50 μg of DNA in Tris buffer $495
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DNA 101023-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV sport
  • Backbone manufacturer
    Life technologies
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 8186
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Glutamate receptor subunit
  • Alt name
    Glutamate receptor 2 GluR2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3504
  • Mutation
    tagged with fluorescent protein
  • Entrez Gene
    Gria2 (a.k.a. GluA2, GluR-K2, GluR2, gluR-B)
  • Promoter CMV
  • Tag / Fusion Protein
    • fluorescent protein

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ecoR1 (unknown if destroyed)
  • 3′ cloning site hind III (unknown if destroyed)
  • 5′ sequencing primer sp6
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pR2Q144c was a gift from Thomas Hughes (Addgene plasmid # 101023 ; http://n2t.net/addgene:101023 ; RRID:Addgene_101023)

  • For your References section:

    Regions of alpha-amino-5-methyl-3-hydroxy-4-isoxazole propionic acid receptor subunits that are permissive for the insertion of green fluorescent protein. Sheridan DL, Robert A, Cho CH, Howe JR, Hughes TE. Neuroscience. 2006 Aug 25;141(2):837-49. Epub 2006 Jun 12. 10.1016/j.neuroscience.2006.04.052 PubMed 16765522
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