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HA-Rab8a-WT
(Plasmid #101048)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101048 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCS2+HA
  • Backbone size w/o insert (bp) 4100
  • Total vector size (bp) 4730
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rab8a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    630
  • Entrez Gene
    RAB8A (a.k.a. MEL, RAB8)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CCCAAGCTTGATTTAG (SP6)
  • 3′ sequencing primer TTATGCTGAGTGATATC (T7)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-Rab8a-WT was a gift from Ken-Ichi Takemaru (Addgene plasmid # 101048 ; http://n2t.net/addgene:101048 ; RRID:Addgene_101048)
  • For your References section:

    Chibby promotes ciliary vesicle formation and basal body docking during airway cell differentiation. Burke MC, Li FQ, Cyge B, Arashiro T, Brechbuhl HM, Chen X, Siller SS, Weiss MA, O'Connell CB, Love D, Westlake CJ, Reynolds SD, Kuriyama R, Takemaru K. J Cell Biol. 2014 Oct 13;207(1):123-37. doi: 10.1083/jcb.201406140. 10.1083/jcb.201406140 PubMed 25313408