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pE-cadherin-GFP-N-WASP deltaVCA
(Plasmid #101283)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101283 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 101283-D50 50 μg of DNA in Tris buffer $495
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DNA 101283-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E-Cadherin-GFP-N-WASP
  • Species
    M. musculus (mouse)
  • Mutation
    delta VCA domain
  • Entrez Gene
    Cdh1 (a.k.a. ARC-1, E-cad, Ecad, L-CAM, UVO, Um)
  • Entrez Gene
    Wasl (a.k.a. 2900021I12Rik, 3110031I02Rik, N-WASP)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

was generated by recombinining mEcad-GFP, a linker5"-GGGTCTACATCTGGATCTGGGAGGCCGGGTTCTGGTGAGGGTTCT3 and N-WASPdeltaVCA into NOT1/Age Iin gfp-N1 vector

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pE-cadherin-GFP-N-WASP deltaVCA was a gift from Alpha Yap (Addgene plasmid # 101283 ; http://n2t.net/addgene:101283 ; RRID:Addgene_101283)

  • For your References section:

    Cortical F-actin stabilization generates apical-lateral patterns of junctional contractility that integrate cells into epithelia. Wu SK, Gomez GA, Michael M, Verma S, Cox HL, Lefevre JG, Parton RG, Hamilton NA, Neufeld Z, Yap AS. Nat Cell Biol. 2014 Feb;16(2):167-78. doi: 10.1038/ncb2900. Epub 2014 Jan 12. 10.1038/ncb2900 PubMed 24413434
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