aCat TL2 (1-697 mTFP1-TSmod-Venus STOP)
(Plasmid #101298)

• Purpose: FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.
• Depositing Lab: Alpha Yap
• Publication: Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867. doi: 10.1016/j.celrep.2017.02.078.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha-Catenin
• Species: M. musculus (mouse)
• Mutation: Contains amino acids 1-698 of alpha-Catenin

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • Annotated Sequence
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The TS module with mTFP1-F40-venusA206K was adapted from Vinculin TS (a kind gift from Martin Schwartz [Grashoff et al., 2010]). The TSmod was cloned in to pEGFP-N1 vector at Xho1 and Not1 restriction sites. For aE-Cat-TS, the mouse N-terminal (1_697 aa) of alpha-catenin was inserted before mTFP1 by in- fusion cloning (Takara Bioscience, Clontech) at the Xho1 restriction site