aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)
PurposeFRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101299||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
MutationContains amino acids 1-698 and 698-906 of alpha-Catenin
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The C-terminal fragment (698-906 aa) of alpha-catenin was cloned after VenusA206K at the Not1 restriction site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907) was a gift from Alpha Yap (Addgene plasmid # 101299 ; http://n2t.net/addgene:101299 ; RRID:Addgene_101299)
For your References section:Mammalian Diaphanous 1 Mediates a Pathway for E-cadherin to Stabilize Epithelial Barriers through Junctional Contractility. Acharya BR, Wu SK, Lieu ZZ, Parton RG, Grill SW, Bershadsky AD, Gomez GA, Yap AS. Cell Rep. 2017 Mar 21;18(12):2854-2867. doi: 10.1016/j.celrep.2017.02.078. 10.1016/j.celrep.2017.02.078 PubMed 28329679