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pRSM13
(Plasmid #101644)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101644 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    meleCFP
  • Insert Size (bp)
    678
  • Tag / Fusion Protein
    • 6XHis (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The fluorescent protein sequence comes from the following publication: Alieva N. O.; Konzen K. A.; Field S. F.; Meleshkevitch E. A.; Hunt M. E.; Beltran-Ramirez V.; Miller D. J.; Wiedenmann J.; Salih A.; Matz M. V. Diversity and Evolution of Coral Fluorescent Proteins. PLoS One 2008, 3, e2680.10.1371/journal.pone.0002680.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSM13 was a gift from Thomas Hughes (Addgene plasmid # 101644 ; http://n2t.net/addgene:101644 ; RRID:Addgene_101644)
  • For your References section:

    Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation. Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, Hughes TE, Drobizhev M. J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25. 10.1021/acs.jpclett.7b00960 PubMed 28530831