pAK22
(Plasmid #101741)

• Purpose: encoding two copies of T. thermophilus SecG for expression of SecYEG complex
• Depositing Lab: Tomoya Tsukazaki
• Publication: Sugano et al PLoS One. 2017 Aug 18;12(8):e0183434. doi: 10.1371/journal.pone.0183434. eCollection 2017.

Backbone

• Vector backbone: pACYCDuet-1
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 3729
• Total vector size (bp): 4344
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Addition of 0.4% Glucose in the media.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Thermus thermophilus HB8 SecG x 2
• Alt name: SecG
• Species: Thermus thermophilus HB8
• Insert Size (bp): 615
• GenBank ID: 3169944
• Promoter: T7
• Tag / Fusion Protein:
  • NONE

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: not used
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry