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pAK22
(Plasmid #101741)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101741 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pACYCDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3729
  • Total vector size (bp) 4344
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Addition of 0.4% Glucose in the media.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Thermus thermophilus HB8 SecG x 2
  • Alt name
    SecG
  • Species
    Thermus thermophilus HB8
  • Insert Size (bp)
    615
  • GenBank ID
    3169944
  • Promoter T7
  • Tag / Fusion Protein
    • NONE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer not used
  • 3′ sequencing primer GCTAGTTATTGCTCAGCGG
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAK22 was a gift from Tomoya Tsukazaki (Addgene plasmid # 101741 ; http://n2t.net/addgene:101741 ; RRID:Addgene_101741)
  • For your References section:

    SecY-SecA fusion protein retains the ability to mediate protein transport. Sugano Y, Furukawa A, Nureki O, Tanaka Y, Tsukazaki T. PLoS One. 2017 Aug 18;12(8):e0183434. doi: 10.1371/journal.pone.0183434. eCollection 2017. PONE-D-17-20168 [pii] PubMed 28820900