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(Plasmid #101856)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101856 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53
  • Backbone size (bp) 6271
  • Modifications to backbone
    Removal of BsaI sites to domesticate backbone for Golden Gate (GG) cloning. Addition of an RFP marker for red-white selection in E. coli. The RFP is flanked by two BsaI sites. GG reaction leads to the loss of the RFP when the gene is inserted. The RFP is expressed in E. coli, allowing for red-white selection of transformants. Antibiotic resistance changed to Ampicillin
  • Vector type
    Fungal expression plasmid, tested in Magnaporthe
  • Selectable markers
    sulfonylurea/chlorimuron ethyl

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Plasmid designed by Helen G. Pennington, and cloning performed by Mark Youles (TSL Synbio)
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid derived from the pCB1532 vector series Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB1532S-RFP was a gift from Sophien Kamoun (Addgene plasmid # 101856 ; ; RRID:Addgene_101856)
  • For your References section:

    Golden-Gate compatible Magnaporthe oryzae protoplast transformation vectors. Pennington HG, Youles M, Kamoun S. Figshare (2017) 10.6084/m9.figshare.5375428.v1