pAUC40
(Plasmid #71298)

• Purpose: (Empty Backbone) Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101
• Depositing Lab: Susanne von Bodman
• Publication: Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. doi: 10.1111/j.1365-2958.2009.06906.x. Epub 2009 Oct 15.

Backbone

• Vector backbone: pKNG101
• Backbone manufacturer: Kaniga, et al Gene. 1991 109: 137-141.
• Backbone size (bp): 8509
• Modifications to backbone: Insertion of Invitrogen Gateway attR-Chloramphenicol resistance cassette from the commercial plasmid conversion kit into the backbone of BamHI/SmaI pairwise-digested plasmid pKNG101 (Kaniga, et al., 1991).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin and Chloramphenicol
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Growth instructions: The specific Pir1 strain used is DB3.1 λpir
• Copy number: Unknown

Cloning Information

• Cloning method: Gateway Cloning

Resource Information

• Supplemental Documents:
  • pAUC40
• A portion of this plasmid was derived from a plasmid made by: Plasmid pAUC40 was synthesized from the backbone of plasmid pKNG101, originally from Kaniga et al., 1991. pAUC40 was developed in the laboratory of Dr. Susanne B. von Bodman at the University of Connecticut.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that there are several discrepancies between Addgene's quality control sequences and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.