pEGFP SNAP47
(Plasmid
#101915)
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PurposeExpresses SNAP47 in mammalian cells
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Depositing Lab
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Depositing OrganizationDelegation INSERM Paris 7
Located in France -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101915 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 101915-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 101915-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP C3
- Backbone size w/o insert (bp) 1341
- Total vector size (bp) 6041
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNAP47
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Alt namesynaptosome associated protein 47, C1orf142, HEL170, SVAP1
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SpeciesH. sapiens (human)
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Insert Size (bp)1341
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GenBank ID116841
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Entrez GeneSNAP47 (a.k.a. C1orf142, ESFI5812, HEL-S-290, HEL170, SNAP-47, SVAP1)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP C
- 3′ sequencing primer EGFP-N
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byProux-Gillardeaux V., Galli T
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 101915-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 101915-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP SNAP47 was a gift from Thierry Galli (Addgene plasmid # 101915 ; http://n2t.net/addgene:101915 ; RRID:Addgene_101915) -
For your References section:
The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs). Kuster A, Nola S, Dingli F, Vacca B, Gauchy C, Beaujouan JC, Nunez M, Moncion T, Loew D, Formstecher E, Galli T, Proux-Gillardeaux V. J Biol Chem. 2015 Nov 20;290(47):28056-69. doi: 10.1074/jbc.M115.666362. Epub 2015 Sep 10. 10.1074/jbc.M115.666362 PubMed 26359495
