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pFastBac_Dual_SNF2L-opt_p48
(Plasmid #102243)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102243 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 9605
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SNF2L
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3177
  • Mutation
    codon optimized for expression
  • Entrez Gene
    SMARCA1 (a.k.a. ISWI, NURF140, SNF2L, SNF2L1, SNF2LB, SNF2LT, SWI, SWI2, hSNF2L)
  • Promoter p10

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma1 (not destroyed)
  • 3′ cloning site Kpn1 (not destroyed)
  • 5′ sequencing primer None
  • 3′ sequencing primer TK-pA-R
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    pRbAp48
  • Alt name
    RBBP4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1278
  • GenBank ID
    NM_005610.2
  • Promoter Polyhedrin

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Hpa1 (destroyed during cloning)
  • 3′ cloning site Sph1 (not destroyed)
  • 5′ sequencing primer Polyhedrin-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac_Dual_SNF2L-opt_p48 was a gift from Joe Landry (Addgene plasmid # 102243 ; http://n2t.net/addgene:102243 ; RRID:Addgene_102243)