pCTcon2-5INS_EV3
(Plasmid
#102293)
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PurposeYeast Surface Display of 5Ins_16_EV3
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Depositing Lab
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102293 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCTCON2
- Backbone size w/o insert (bp) 6330
- Total vector size (bp) 6846
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEvolved Insulin binding scFV
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Insert Size (bp)723
- Promoter GAL1
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- Myc (C terminal on backbone)
- 3x(G4S) Linker (N terminal on backbone)
- G3S Linker (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer GAL1
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCTcon2-5INS_EV3 was a gift from Sarel Fleishman (Addgene plasmid # 102293 ; http://n2t.net/addgene:102293 ; RRID:Addgene_102293) -
For your References section:
Principles for computational design of binding antibodies. Baran D, Pszolla MG, Lapidoth GD, Norn C, Dym O, Unger T, Albeck S, Tyka MD, Fleishman SJ. Proc Natl Acad Sci U S A. 2017 Oct 10;114(41):10900-10905. doi: 10.1073/pnas.1707171114. Epub 2017 Sep 25. 10.1073/pnas.1707171114 PubMed 28973872
