pTR47m4-GFP
(Plasmid
#102436)
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PurposeFormaldehyde biosensor plasmid with sGFP reporter and modified frm promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102436 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTR47m4
- Total vector size (bp) 4328
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor inducible expression of frmR, use in a strain with tetR (e.g. S1030)
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert nameFormaldehyde repressor protein (frmR)
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Alt namefrmR
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SpeciesE. coli
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Insert Size (bp)276
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GenBank IDNP_414891.4
Gene/Insert 2
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Gene/Insert nameSuperfolder GFP
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Alt namesGFP
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Insert Size (bp)717
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTR47m4-GFP was a gift from Gregory Stephanopoulos (Addgene plasmid # 102436 ; http://n2t.net/addgene:102436 ; RRID:Addgene_102436) -
For your References section:
Development of a Formaldehyde Biosensor with Application to Synthetic Methylotrophy. Woolston BM, Roth T, Kohale I, Liu D, Stephanopoulos G. Biotechnol Bioeng. 2017 Sep 16. doi: 10.1002/bit.26455. 10.1002/bit.26455 PubMed 28921510
