S449F EPHA3 pcDNA3.1
(Plasmid
#102522)
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PurposeExpression of EPHA3 in mammalian cells
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Depositing Lab
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 102522 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1(+)/myc-His B
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPHA3
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SpeciesH. sapiens (human)
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MutationS449F
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Entrez GeneEPHA3 (a.k.a. EK4, ETK, ETK1, HEK, HEK4, TYRO4)
- Promoter CMV
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Tag
/ Fusion Protein
- myc-6xhis (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
S449F EPHA3 pcDNA3.1 was a gift from Jin Chen (Addgene plasmid # 102522) -
For your References section:
Effects of cancer-associated EPHA3 mutations on lung cancer. Zhuang G, Song W, Amato K, Hwang Y, Lee K, Boothby M, Ye F, Guo Y, Shyr Y, Lin L, Carbone DP, Brantley-Sieders DM, Chen J. J Natl Cancer Inst. 2012 Aug 8;104(15):1182-97. doi: 10.1093/jnci/djs297. Epub 2012 Jul 24. 10.1093/jnci/djs297 PubMed 22829656