PurposeBacterial expression of EYFP fused to the cell penetrating peptide TAT with a GST tag for purification
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102541||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4984
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Promoter tac
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
TAT-peptide is N-terminal to, and in frame with, EYFP (between GST and EYFP); BL-21 cells are used for protein expression
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SRpHi1 was a gift from Stefan Hell (Addgene plasmid # 102541 ; http://n2t.net/addgene:102541 ; RRID:Addgene_102541)
For your References section:SRpHi ratiometric pH biosensors for super-resolution microscopy. Richardson DS, Gregor C, Winter FR, Urban NT, Sahl SJ, Willig KI, Hell SW. Nat Commun. 2017 Sep 18;8(1):577. doi: 10.1038/s41467-017-00606-4. 10.1038/s41467-017-00606-4 [pii] PubMed 28924139